Antioxidant Capacity of Binuclear Cu(II)-Cyclophanes, Insights from Two Synthetic Bioactive Molecules

作者：Sugich Miranda Rocio; Sotelo Mundo Rogerio R; Silva Campa Erika; Hernandez Jesus; Gonzalez Aguilar Gustavo A; Velazquez Contreras Enrique F^*

来源：Journal of Biochemical and Molecular Toxicology, 2010, 24(6): 379-383.

DOI：10.1002/jbt.20350

• Cyclophane
• Macrocyclic
• Copper
• Antioxidant capacity
• TEAC

The compounds 2,9,25,32-tetraoxo-4,7,27,30-tetrakis(carboxymethyl)-1,4,7,10,24,27,30,33- octaaza-17,40-dioxa[10.1.10.1]paracyclophane and 2,9, 25,32-tetraoxo-4,7,27,30-tetrakis(carboxymethyl)-1,4,7, 10,24,27,30,33-octaaza[10.1.10.1]paracyclophane binuclear copper complexes (Cu(2)PO and Cu(2)PC, respectively) were studied by determining their antioxidant capacity using the TROLOX equivalent antioxidant capacity (TEAC) assay, and their cytotoxicity on cultured cells, as well as the superoxide dismutase (SOD)-like activity. Cu(2)PO had an antioxidant capacity (0.1 g eq TROLOX mol(-1)) within the order of magnitude of ascorbic acid, and both, Cu(2)PO and Cu2PC were nontoxic to cultured peripheral mononuclear blood cells. The SOD-like activity was evaluated using the nitroblue tetrazolium assay, and both compounds presented an excellent activity: for Cu(2)PO, the IC(50) was 52 nM and for Cu(2)PC an IC(50) of 0.5 mu M was obtained comparable to CuZn SOD IC(50) 17 nM (Fernandes et al., J Inorg Biochem 2007;101:849-858). These results suggest that synthetic binuclear macrocycles are good candidates to be used as synthetic bioactive molecules with applications in biomedicine.

• 出版日期2010