Nano-structured arrays for multiplex analyses and Lab-on-a-Chip applications

作者：Dixit Chandra K; Kaushik Ajeet^*

来源：Biochemical and Biophysical Research Communications, 2012, 419(2): 316-320.

DOI：10.1016/j.bbrc.2012.02.018

• Nanosphere lithography
• Microfluidics
• Lab-on-a-Chip
• Antibody
• ELISA
• Immunofluorescence

Nanospheres lithographic (NSL) method has been used to fabricate nano-structured arrays (NAs) of hexagonally close-packed gold (Au) using polystyrene beads [PS, diameter similar to 300 nm] as mask. The developed NA was incorporated with a customized and cheap microfluidics system to demonstrate its applicability as an alternative easy and efficient platform for multiplex analysis and Lab-on-a-Chip applications. The chip functionality was demonstrated with horseradish peroxidase (HRP) and anti-HRP antibody as model for recognition system. The enzyme-linked immunosorbent assay (ELISA) performed on fabricated protein biochip had a detection limit 100 pg/mL for HRP. The antibody chip was also checked for the shelf-life and it was found that these chips could be stored for 50 days when stored at 4 degrees C without any significant loss of activity. Therefore, NAs based protein biochip with the correct microfluidics could find huge potential application in diagnostics and biosensing technology.

• 出版日期2012-3-9