A modified technique for efficient radiolabefing of (68)Gacitrate from a SnO2-based Ge-68/Ga-68 generator for better infection imaging

作者：Vorster Mariza; Mokaleng Brenda; Sathekge Mike M^*; Ebenhan Thomas

来源：Hellenic Journal of Nuclear Medicine, 2013, 16(3): 193-198.

• SnO2-based Ga-68/Ge-68 generator
• Ga-68-ACD-A
• Ga-68-citrate
• PET/CT infection imaging

Our aim was to develop a practical method to prepare Ga-68-citrate using a SnO2-based Ge-68/Ga-68 generator and evaluate its use in infection imaging. Ga-68-citrate synthesis was performed in a straight forward, quantitative, one-step-aseptic procedure; an amended labeling method was applied using ACD-A buffered citrate as a precursor. We have studied 36 patients (12 with TB, 12 with various cancers, 12 with inflammation) and 10 controls. Study participants were imaged on a Siemens Biograph 40 PET/CT scanner 60min post intravenous injection. Our results showed: 90%-95% Ga-68-yield was obtained and subsequently used at 324-527MBq to perform three to four parallel Ga-68-citrate syntheses. Ga-68-citrate of 96%-99% was yielded after 10min incubation. The radiochemical purity was %26gt;99% with a pH value of 4.0-4.5. All other quality control requirements were met. The (68)Gacitrate stability was %26gt;96%. The final product was sterile; pyrogen and solvent-free, with very low Ge-68-levels, with 191 +/- 33MBq in 6.6 +/- 2.8mL. High quality images were obtained at 60min post injection of 185MBq of Ga-68-citrate. In conclusion, a fast, direct and cheap method with a quantitative preparation of Ga-68-citrate was described. We reported on the adaptations needed when using a SnO2-based Ge-68/Ga-68 generator and ACD-A buffered citrate as a precursor. This method allowed for multiple patient productions from one generator elution, with 300MBq/patient of Ga-68-citrate produced in less than 30min and excellent labeling reproducibility for routine infection imaging.

• 出版日期2013-12