Macrophage reactivity to different polymers demonstrates particle size- and material-specific reactivity: PEEK-OPTIMA (R) particles versus UHMWPE particles in the submicron, micron, and 10 micron size ranges

作者：Hallab Nadim James^*; McAllister Kyron; Brady Mark; Jarman Smith Marcus

来源：Journal of Biomedical Materials Research Part B: Applied Biomaterials , 2012, 100B(2): 480-492.

DOI：10.1002/jbm.b.31974

• MG-63 osteoblasts
• THP-1 macrophages
• fibroblasts
• particles
• PEEK
• UHMWPE

Biologic reactivity to orthopedic implant debris is generally the main determinant of long-term clinical performance where released polymeric particles of Ultra-high molecular weight polyethylene (UHMWPE) remain the most prevalent debris generated from metal-on-polymer bearing total joint arthroplasties. Polymeric alternatives to UHMWPE such as polyetherether-ketone (PEEK) may have increased wear resistance but the bioreactivity of PEEK-OPTIMA particles on peri-implant inflammation remains largely uncharacterized. We evaluated human monocyte/macrophage responses (THP-1s and primary human) when challenged by PEEK-OPTIMA, UHMWPE, and X-UHMWPE particles of three particle sizes (0.7 um, 2 um, and 10 um) at a dose of 20 particles-per-cell at 24- and 48-h time points. Macrophage responses were measured using cytotoxicity assays, viability assays, proliferation assays and cytokine analysis (IL-1b, IL-6, IL-8, MCP-1, and TNF-a). In general, there were no significant differences between PEEK-OPTIMA, UHMWPE, and X-UHMWPE particles on macrophage viability or proliferation. However, macrophages demonstrated greater cytotoxicity responses to UHMWPE and X-UHMWPE than to PEEK-OPTIMA at 24 and 48 h, where 0.7 mu m-UHMWPE particles produced the highest amount of cytotoxicity. Particles of X-UHMWPE more than PEEK-OPTIMA and UHMWPE induced IL-1 beta, IL-6, MCP-1, and TNF-a at 24 h, p %26lt; 0.05 (no significant differences at 48 h). On average, cytokine production was more adversely affected by larger 10 mu m particles than by 0.7 and 2 mu m sized particles. While limitations of in vitro analysis apply to this study, PEEK-OPTIMA particles were more biocompatible than UHMWPE particles, in that they induced less inflammatory cytokine responses and thus, in part, demonstrates that PEEK-OPTIMA implant debris does not represent an increased inflammatory risk over that of UHMWPE.

• 出版日期2012-2