Accumulation of mitochondrial DNA deletions within dopaminergic neurons triggers neuroprotective mechanisms

作者：Perier Celine; Bender Andreas; Garcia Arumi Elena; Jesus Melia Ma; Bove Jordi; Laub Christoph; Klopstock Thomas; Elstner Matthias; Mounsey Ross B; Teismann Peter; Prolla Tomas; Andreu Antoni L; Vila Miquel^*

来源：Brain, 2013, 136(8): 2369-2378.

DOI：10.1093/brain/awt196

• Parkinson disease
• mitochondria
• mtDNA deletions
• neurodegeneration

Acquired alterations in mitochondrial DNA are believed to play a pathogenic role in Parkinson%26apos;s disease. In particular, accumulation of mitochondrial DNA deletions has been observed in substantia nigra pars compacta dopaminergic neurons from patients with Parkinson%26apos;s disease and aged individuals. Also, mutations in mitochondrial DNA polymerase gamma result in multiple mitochondrial DNA deletions that can be associated with levodopa-responsive parkinsonism and severe substantia nigra pars compacta dopaminergic neurodegeneration. However, whether mitochondrial DNA deletions play a causative role in the demise of dopaminergic neurons remains unknown. Here we assessed the potential pathogenic effects of mitochondrial DNA deletions on the dopaminergic nigrostriatal system by using mutant mice possessing a proofreading-deficient form of mitochondrial DNA polymerase gamma (POLG(D257A)), which results in a time-dependent accumulation of mitochondrial DNA deletions in several tissues, including the brain. In these animals, we assessed the occurrence of mitochondrial DNA deletions within individual substantia nigra pars compacta dopaminergic neurons, by laser capture microdissection and quantitative real-time polymerase chain reaction, and determined the potential deleterious effects of such mitochondrial DNA alterations on mitochondrial function and dopaminergic neuronal integrity, by cytochrome c oxidase histochemistry and quantitative morphology. Nigral dopaminergic neurons from POLG(D257A) mice accumulate mitochondrial DNA deletions to a similar extent (similar to 40-60%) as patients with Parkinson%26apos;s disease and aged individuals. Despite such high levels of mitochondrial DNA deletions, the majority of substantia nigra pars compacta dopaminergic neurons from these animals did not exhibit mitochondrial dysfunction or degeneration. Only a few individual substantia nigra pars compacta neurons appeared as cytochrome c oxidase-negative, which exhibited higher levels of mitochondrial DNA deletions than cytochrome c oxidase-positive cells (60.38 +/- 3.92% versus 45.18 +/- 2.83%). Survival of dopaminergic neurons in POLG(D257A) mice was associated with increased mitochondrial DNA copy number, enhanced mitochondrial cristae network, improved mitochondrial respiration, decreased exacerbation of mitochondria-derived reactive oxygen species, greater striatal dopamine levels and resistance to parkinsonian mitochondrial neurotoxins. These results indicate that primary accumulation of mitochondrial DNA deletions within substantia nigra pars compacta dopaminergic neurons, at an extent similar to that observed in patients with Parkinson%26apos;s disease, do not kill dopaminergic neurons but trigger neuroprotective compensatory mechanisms at a mitochondrial level that may account for the high pathogenic threshold of mitochondrial DNA deletions in these cells.

• 出版日期2013-8