Adsorption of human serum albumin onto PVA-coated affinity microporous PTFE capillary

作者：Jin Gu^*; Yao Qi-zhi; Zhang Lei

来源：Chemical Research in Chinese Universities, 2008, 24(2): 154-161.

DOI：10.1016/S1005-9040(08)60032-X

• albumin adsorption
• cibacron blue F3GA
• PVA
• PTFE
• plasma

Affinity dye-ligand Cibacron Blue F3GA(CB F3GA) was covalently coupled with poly(vinyl alcohol)(PVA) coated on the inner surface of microporous poly(tetra-fluoroethylene)(MPTFE) membranous capillary. The PVA-coated PTFE capillary surface was characterized by XPS and FESEM. The grafting degree of PVA and the amount of CB F3GA immobilized onto the membranous capillary were 23.5 mg/g and 89.6 mu mol/g, respectively. These dyed membranous capillaries were chemically and mechanically stable, and could be reproducibly prepared. Human serum albumin(HSA) was selected as model protein. The saturation adsorbance of the dye attached membranous capillary was 85.3 mg HSA/g, while the capacity of non-specific adsorption for HSA was less than 0.3 mg/g.

• 出版日期2008-3
• 单位中国科学技术大学