Mitochondrial connexin 43 impacts on respiratory complex I activity and mitochondrial oxygen consumption

作者：Boengler Kerstin; Ruiz Meana Marisol; Gent Sabine; Ungefug Elvira; Soetkamp Daniel; Miro Casas Elisabet; Cabestrero Alberto; Fernandez Sanz Celia; Semenzato Martina; Di Lisa Fabio; Rohrbach Susanne; Garcia Dorado David; Heusch Gerd; Schulz Rainer^*

来源：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2012, 16(8): 1649-1655.

DOI：10.1111/j.1582-4934.2011.01516.x

• Connexin 43
• 18a glycyrrhetinic acid
• mitochondria
• respiration

Connexin 43 (Cx43) is present at the sarcolemma and the inner membrane of cardiomyocyte subsarcolemmal mitochondria (SSM). Lack or inhibition of mitochondrial Cx43 is associated with reduced mitochondrial potassium influx, which might affect mitochondrial respiration. Therefore, we analysed the importance of mitochondrial Cx43 for oxygen consumption. Acute inhibition of Cx43 in rat left ventricular (LV) SSM by 18a glycyrrhetinic acid (GA) or Cx43 mimetic peptides (Cx43-MP) reduced ADP-stimulated complex I respiration and ATP generation. Chronic reduction of Cx43 in conditional knockout mice (Cx43Cre-ER(T)/fl + 4-OHT, 510% of Cx43 protein compared with control Cx43fl/fl mitochondria) reduced ADP-stimulated complex I respiration of LV SSM to 47.8 +/- 2.4 nmol O2/min.*mg protein (n = 8) from 61.9 +/- 7.4 nmol O2/min.*mg protein in Cx43fl/fl mitochondria (n = 10, P %26lt; 0.05), while complex II respiration remained unchanged. The LV complex I activities (% of citrate synthase activity) of Cx43Cre-ER(T)/fl+4-OHT mice (16.1 +/- 0.9%, n = 9) were lower than in Cx43fl/fl mice (19.8 +/- 1.3%, n = 8, P %26lt; 0.05); complex II activities were similar between genotypes. Supporting the importance of Cx43 for respiration, in Cx43-overexpressing HL-1 cardiomyocytes complex I respiration was increased, whereas complex II respiration remained unaffected. Taken together, mitochondrial Cx43 is required for optimal complex I activity and respiration and thus mitochondrial ATP-production.

• 出版日期2012-8