Ultrastructural analysis of beta-lapachone-induced surface membrane damage in male adult Schistosoma mansoni BH strain worms

作者：Aires Andre de Lima; Pessoa Azevedo Ximenes Eulalia Camelo; Ramos Silva Renata Alexandre; Barbosa Vanessa Xavier; da Silva Goes Alexandre Jose; Peixoto Christina Alves; Oliveira Souza Valdenia Maria; Pessoa de Azevedo Albuquerque Monica Camelo

来源：Experimental Parasitology, 2014, 142: 83-90.

DOI：10.1016/j.exppara.2014.04.010

• beta-Lapachone
• Schistosoma mansoni
• Ultrastructural analysis
• Tegument

The present study provides, for the first time, conclusions on the in vitro schistosomicidal properties of beta-lap. Adult male Schistosoma mansoni worms of the BH strain were used for the study. Motility, mortality, cell viability and alterations in the tegument were employed as schistosomicidal parameters. Alterations in motility were observed 6 h after incubation in concentrations of 50 and 100 mu M. beta-lap decreased significantly the worm viability, reducing the formation of formazan in 17.7%, 27.4% and 54.8% at concentrations of 25, 50 and 100 mu M, respectively. Mortality in concentrations of 50 and 100 mu M was of 67% and 100%, respectively, after 24 h. The death of the parasite was preceded by progressive surface membrane damage, characterized by tegument peeling, spine reduction and erosion, blister formation and rupture, and the emergence of holes. In addition to this, in the anterior portion, intense general edema, areas of cracking with a wrinkled surface, furrows and a fibrous appearance were also observed. The results of the present study thus provide a sound basis for further in-depth studies of the schistosomicidal properties of mu-lap, both in the laboratory and in the field.

• 出版日期2014-7