A novel USP9Y polymorphism allowing a rapid and unambiguous classification of Bos taurus Y chromosomes into haplogroups

作者：Bonfiglio S; De Gaetano A; Tesfaye K; Grugni V; Semino O; Ferretti L^*

来源：Animal Genetics, 2012, 43(5): 611-613.

DOI：10.1111/j.1365-2052.2012.02328.x

• cattle
• haplogroup classification
• phylogeny
• Y chromosome
• Y-specific biallelic markers

A new sequence-tagged site (STS) was identified within intron 26 of the bovine USP9Y gene, showing an 81-base pair insertion (g.76439_76440ins81 in sequence with GenBank accession FJ195366) able to distinguish Y2 and Y3 Bos Y haplogroups from Y1. Moreover, four Y3-specific sequence variants allow a distinction from haplogroup Y2. The typing of a Bison bison Y chromosome indicates that the ancestral allele for the USP9Y 81-bp insertion is the short Y1 version. The results from typing the new STS in 1230 cattle Y chromosomes are fully consistent with their classification through standard methods. Thanks to the newly identified STS, it is now possible to assign cattle Y chromosomes to the currently known haplogroups using a single marker.

• 出版日期2012-10