Dietary glycerol for quail: association between productive performance and COX III mRNA expression

作者：Silva S C C^*; Gasparino E; Batista E; Tanamati F; Vesco A P D; Lala B; de Oliveira D P

来源：Genetics and Molecular Research, 2016, 15(2): 15027435.

DOI：10.4238/gmr.15027435

• Electron transport chain
• Gene expression
• Oxidative phosphorylation
• Energy metabolism

This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake were evaluated. COX III mRNA expression in breast muscle was higher in the group fed with 12% glycerol (0.863 AU); no differences were observed in the expression of this gene between the muscle of animals fed diets without glycerol (0.357 AU) and 8% glycerol (0.415 AU). Quails that showed greater COX III mRNA expression also showed the lowest feed efficiency. These results show that there is a difference in COX III mRNA expression in breast muscle of 28-day-old quail fed diets different concentrations of glycerol.

• 出版日期2016