MicroRNA 132 alters sleep and varies with time in brain

作者：Davis Christopher J^*; Clinton James M; Taishi Ping; Bohnet Stewart G; Honn Kimberly A; Krueger James M

来源：Journal of Applied Physiology, 2011, 111(3): 665-672.

DOI：10.1152/japplphysiol.00517.2011

• delta power
• electroencephologram
• slow-wave activity
• RNA interference
• non-rapid-eye-movement sleep

Davis CJ, Clinton JM, Taishi P, Bohnet SG, Honn KA, Krueger JM. MicroRNA 132 alters sleep and varies with time in brain. J Appl Physiol 111: 665-672, 2011. First published June 30, 2011; doi: 10.1152/japplphysiol.00517.2011.-MicroRNA (miRNA) levels in brain are altered by sleep deprivation; however, the direct effects of any miRNA on sleep have not heretofore been described. We report herein that intracerebroventricular application of a miRNA-132 mimetic (preMIR-132) decreased duration of non-rapid-eye-movement sleep (NREMS) while simultaneously increasing duration of rapid eye movement sleep (REMS) during the light phase. Further, preMIR-132 decreased electroencephalographic (EEG) slow-wave activity (SWA) during NREMS, an index of sleep intensity. In separate experiments unilateral supracortical application of preMIR-132 ipsilaterally decreased EEG SWA during NREMS but did not alter global sleep duration. In addition, after ventricular or supracortical injections of preMIR-132, the mimetic-induced effects were state specific, occurring only during NREMS. After local supracortical injections of the mimetic, cortical miRNA-132 levels were higher at the time sleep-related EEG effects were manifest. We also report that spontaneous cortical levels of miRNA-132 were lower at the end of the sleep-dominant light period compared with at the end of the dark period in rats. Results suggest that miRNAs play a regulatory role in sleep and provide a new tool for investigating sleep regulation.

• 出版日期2011-9