Uncovering the Forgotten Effect of Superior Cervical Ganglia on Pupil Diameter in Subarachnoid Hemorrhage: an Experimental Study

作者：Onen Mehmet Resid; Yilmaz Ilhan; Ramazanoglu Leyla; Aydin Mehmet Dumlu^*; Keles Sadullah; Baykal Orhan; Aydin Nazan; Gundogdu Cemal

来源：Turkish Neurosurgery, 2018, 28(1): 48-55.

DOI：10.5137/1019-5149.JTN.16867-16.3

• Subarachnoid hemorrhage
• Pupil diameter
• Superior cervical ganglia
• Sympathetic innervation
• Light reflex

AIM: To investigate the relationship between neuron density of the superior cervical sympathetic ganglia and pupil diameter in subarachnoid hemorrhage.
MATERIAL and METHODS: This study was conducted on 22 rabbits; 5 for the baseline control group, 5 for the SHAM group and 12 for the study group. Pupil diameters were measured via sunlight and ocular tomography on day 1 as the control values. Pupil diameters were re-measured after injecting 0.5 cc saline to the SHAM group, and autologous arterial blood into the cisterna magna of the study group. After 3 weeks, the brain, superior cervical sympathetic ganglia and ciliary ganglia were extracted with peripheral tissues bilaterally and examined histopathologically. Pupil diameters were compared with neuron densities of the sympathetic ganglia and ciliary ganglia which were examined using stereological methods.
RESULTS: Baseline values were; normal pupil diameter 7.180 +/- 620 mu m and mean neuron density of the superior cervical sympathetic ganglia 6.321 +/- 510/mm(3), degenerated neuron density of ciliary ganglia was 5 +/- 2/mm(3) after histopathological examination in the control group. These values were measured as 6.850 +/- 578 mu m, 5.950 +/- 340/mm(3) and 123 +/- 39/mm(3) in the SHAM group and 9.910 +/- 840 mu m, 7.950 +/- 764/mm(3) and 650 +/- 98/mm(3) in the study group. A linear relationship was determined between neuron density of the superior cervical sympathetic ganglia and pupil diameters (p<0.005). Degenerated ciliary ganglia neuron density had an inverse effect on pupil diameters in all groups (p<0.0001).
CONCLUSION: Highly degenerated neuron density of the ciliary ganglion is not responsible for pupil dilatation owing to parasympathetic pupilloconstrictor palsy, but high neuron density of the pupillodilatatory superior cervical sympathetic ganglia should be considered an important factor for pupil dilatation.

• 出版日期2018