Paris saponin VII suppressed the growth of human cervical cancer Hela cells

作者：Zhang Wenjie; Zhang Dian^*; Ma Xi; Liu Zhaoyang; Li Fang; Wu Dongna

来源：European Journal of Medical Research, 2014, 19(1): 41.

DOI：10.1186/2047-783X-19-41

• Paris saponin VII (PS VII)
• Cervical cancer
• Proliferation
• Apoptosis

Background: Saponins of several herbs are known to induce apoptosis in many cancer cells. The present study aimed to investigate the growth inhibitory effect of Paris saponin VII (PS VII), a kind of steroidal saponins from Chonglou (Rhizoma Paridis Chonglou), on the human cervical cancer cell line Hela and the relative molecular mechanisms.
Methods: Hela cells were exposed to different concentrations of PS VII (1 to 100 M). Inhibition of cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-ethynyl-2'-deoxyuridine (EdU) assays. The amount of apoptotic cells was evaluated by flow cytometric analysis. And the protein level of cleaved caspase-3, cleaved caspase-9, Bax, and Bcl-2 was evaluated by Western blot.
Results: The half maximal inhibitory concentration (IC50) value of PS VII for the growth inhibition of Hela cells was 2.62 +/- 0.11 mu M. PS VII increased the expression of caspase-3, caspase-9, and Bax while decreased that of Bcl-2, suggesting that PS VII may induce apoptosis through intrinsic apoptotic ways.
Conclusions: These data indicate that PS VII has the potential for the treatment of cervical cancer.

• 出版日期2014-8-15
• 单位西安医学院