In vivo sampling with solid phase microextraction

作者：Musteata Florin Marcel; Pawliszyn Janusz^*

来源：Journal of Biochemical and Biophysical Methods, 2007, 70(2): 181-193.

DOI：10.1016/j.jbbm.2006.07.006

• in vivo sampling
• solid phase microextraction
• free concentrations
• drug analysis
• volatile emissions

This review discusses the most recent developments and future challenges in the application of solid phase microextraction (SPME) for sampling of live biological samples. The emphasis is placed on applications of fiber SPME for analysis of volatile emissions and drugs in biological fluids. The method development section highlights the main parameters that need to be considered in the case of in vivo experiments: extraction techniques, selection of extraction phases, calibration procedures, determination of free concentrations, and automation.

• 出版日期2007-3-10