Acellular human corneal matrix sheets seeded with human adipose-derived mesenchymal stem cells integrate functionally in an experimental animal model

作者：Alio del Barrio Jorge L; Chiesa Massimo; Garagorri Nerea; Garcia Urquia Nerea; Fernandez Delgado Jorge; Bataille Laurent; Rodriguez Alejandra; Arnalich Montiel Francisco; Zarnowski Tomasz; Alvarez de Toledo Juan P; Alio Jorge L; De Miguel Maria P^*

来源：Experimental Eye Research, 2015, 132: 91-100.

DOI：10.1016/j.exer.2015.01.020

• Adipose derived stem cells
• Cornea
• Decellularization
• Recellularization
• Lamellar corneal transplant
• Corneal stroma regeneration
• Tissue engineering

Purpose: To evaluate the in vivo biocompatibility of grafts composed of sheets of decellularized human corneal stroma with or without the recellularization of human adipose derived adult stem cells (h-ADASC) into the rabbit cornea.
Methods: Sheets of human corneal stroma of 90 gm thickness were decellularized, and their lack of cytotoxicity was assayed. The recellularization was achieved by the injection of 2 x 10(5) labeled h-ADASC in the graft followed by five days of cell culture. The grafts were implanted in vivo into a stromal pocket at 50% depth. After a triple-masked three-month follow-up, the animals were euthanized and the bio-integration of the graft, the viability of the stem cells and the expression of keratocan (human keratocyte-specific protein) were assessed.
Results: The decellularized stromal sheets showed an intact extracellular matrix with a decellularization rate of 92.8% and an excellent recellularization capacity in vitro with h-ADASC. A complete and stable graft transparency was observed during the full follow-up, with absence of any clinical sign of rejection. The postmortem analysis demonstrated the survival of the transplanted human stem cells inside the graft and their differentiation into functional keratocytes, as assessed by the expression of human keratocan.
Conclusions: We report a new model of lamellar keratoplasty that requires only a simple and safe procedure of liposuction and a donor allogeneic cornea to provide an optically transparent autologous stromal graft with excellent biocompatibility and integration into the host tissue in a rabbit model.

• 出版日期2015-3