A new on-axis multimode spectrometer for the macromolecular crystallography beamlines of the Swiss Light Source

作者：Owen Robin L; Pearson Arwen R; Meents Alke; Boehler Pirmin; Thominet Vincent; Schulze Briese Clemens^*

来源：Journal of Synchrotron Radiation, 2009, 16(2): 173-182.

DOI：10.1107/S0909049508040120

• single-crystal microspectrophotometry
• kinetic crystallography
• structural enzymology
• radiation damage
• macromolecular crystallography
• complementary techniques

X-ray crystallography at third-generation synchrotron sources permits tremendous insight into the three-dimensional structure of macromolecules. Additional information is, however, often required to aid the transition from structure to function. In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes. Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source. The continuing development of the spectrometer is also outlined.

• 出版日期2009-3