Differential protein expression during colonic adaptation in ultra-short bowel rats

作者：Jiang, Hai-Ping; Chen, Tao; Yan, Guang-Rong^*; Chen, Dan

来源：World Journal of Gastroenterology, 2011, 17(20): 2572-2579.

DOI：10.3748/wjg.v17.i20.2572

• Ultra-short bowel syndrome
• Enteral nutrition
• Colon adaptation
• Proteomics

AIM: To investigate the proteins involved in colonic adaptation and molecular mechanisms of colonic adaptation in rats with ultra-short bowel syndrome (USBS).
METHODS: Sprague Dawley rats were randomly assigned to three groups: USBS group (10 rats) undergoing an approximately 90%-95% small bowel resection; sham-operation group (10 rats) undergoing small bowel transaction and anastomosis; and control group (ten normal rats). Colon morphology and differential protein expression was analyzed after rats were given post-surgical enteral nutrition for 21 d. Protein expression in the colonic mucosa was analyzed by two-dimensional electrophoresis (2-DE) in all groups. Differential protein spots were detected by ImageMaster 2D Platinum soft-ware and were further analyzed with matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight-mass spectrometric (MALDI-TOF/TOF-MS) analysis.
RESULTS: The colonic mucosal thickness significantly increased in the USBS group compared with the control group (302.1 +/- 16.9 mu m vs 273.7 +/- 16.0 mu m, P < 0.05). There was no statistically significant difference between the sham-operation group and control group (P > 0.05). The height of colon plica markedly improved in USBS group compared with the control group (998.4 +/- 81.2 mu m vs 883.4 +/- 39.0 mu m, P < 0.05). There was no statistically significant difference between the sham-operation and control groups (P > 0.05). A total of 141 differential protein spots were found in the USBS group. Forty-nine of these spots were down-regulated while 92 protein spots were up-regulated by over 2-folds. There were 133 differential protein spots in USBS group. Thirty of these spots were down-regulated and 103 were up-regulated. There were 47 common differential protein spots among the three groups, including 17 down-regulated protein spots and 30 up-regulated spots. Among 47 differential spots, eight up-regulated proteins were identified by MALDI-TOF/TOF-MS. These proteins were previously reported to be involved in sugar and fat metabolism, protein synthesis and oxidation reduction, which are associated with colonic adaption.
CONCLUSION: Eight proteins f

• 出版日期2011-5-28
• 单位暨南大学