Multi wall carbon nanotubes induce oxidative stress and cytotoxicity in human embryonic kidney (HEK293) cells

作者：Reddy Anreddy Rama Narsimha; Reddy Yellu Narsimha^*; Krishna Devarakonda Rama; Himabindu Vurimindi

来源：Toxicology, 2010, 272(1-3): 11-16.

DOI：10.1016/j.tox.2010.03.017

• Carbon nanotubes
• MWCNT
• HEK293 cells
• Oxidative stress
• Cytotoxicity
• In vitro

The present study was aimed at evaluating the potential toxicity and the general mechanism involved in multi wall carbon nanotubes (MWCNT)-induced cytotoxicity using human embryonic kidney cell line (HEK293) cells Two multi wall carbon nanotubes (coded as MWCNT1, size 90-150 nm and MWCNT2, size 60-80 nm) used in this study are MWCNT1 (produced by the electric arc method and size of the nanotubes was 90-150 nm) and MWCNT2 (produced by the chemical vapor deposition method with size of 60-80 nm) To elucidate the possible mechanisms of MWCNT Induced cytotoxicity, cell viability, mitochondrial MTT assay), cell membrane damage (LDH assay), reduced glutathione (GSH), interleukin-8 (IL-8) and lipid peroxidation levels were quantitatively assessed under carbon nanotubes exposed (48 h) conditions. Exposure of different sizes of two carbon nanotubes at dosage levels between 3 and 300 mu g/ml decreased cell viability in a concentration dependent manner. The IC(50) values (concentration of nanoparticles to induce 50% cell mortality) of two (MWCNT1, MWCNT2) nanoparticles were found as 42.10 and 36 95 mu g/ml Exposure of MWCNT (10-100 mu g/ml) to HEK cells resulted in concentration dependent cell membrane damage (as indicated by the increased levels of LDH), increased production of IL-8, increased TBARS and decreased intracellular glutathione levels. The cytotoxicity and oxidative stress was significantly more in MWCNT2 exposed cells than MWCNT1. In summary, exposure of carbon nanotubes resulted in a concentration dependent cytotoxicity in cultured HEK293 cells that was associated with increased oxidative stress.

• 出版日期2010-6-4