Molecular characterization and expression analysis of the β-actin gene from the ridgetail white prawn Exopalaemon carinicauda

作者：Liang, J. P.; Wang, Y.; Ge, Q. Q.; Li, J. T.; Liu, P.; Li, J.; Nie, G. X.

来源：Genetics and Molecular Research, 2016, 15(2): 15026872.

DOI：10.4238/gmr.15026872

• Exopalaemon carinicauda
• beta-actin
• Ovarian development
• Internal control
• Gene expression

Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ec beta-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ec beta-actin was 1335 bp long, comprising a 1131-bp ORF encoding 376 amino acids, a 65-bp 5'-UTR, and a 139-bp 3'-UTR with a poly(A) tail. The A + T content was approximately 79% in the 3'-UTR of the Ec beta-actin mRNA. The 3'-UTR contained two repeats of the AUUUA motif. The putative protein Ec beta-actin showed high identity (97-99%) with other actins

• 出版日期2016
• 单位河南师范大学; 中国水产科学研究院