A highly selective and sensitive Tb3+-acetylacetone photo probe for the assessment of acetazolamide in pharmaceutical and serum samples

作者：Youssef A O^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 195: 47-52.

DOI：10.1016/j.saa.2018.01.047

• Acetazolamide
• Luminescence intensity
• Energy transfer
• Terbium sensitized
• Photo probe

A novel, simple, sensitive and selective spectrofluorimetric method was developed for the determination of Acetazolamide in pharmaceutical tablets and serum samples using photo probe Tb3+-ACAC. The Acetazolamide can remarkably quench the luminescence intensity of Tb3+-ACAC complex in DMSO at pH 6.8 and lambda(ex) = 350 nm. The quenching of luminescence intensity of Tb3+-ACAC complex especially the electrical band at lambda(em) = 545 nm is used for the assessment of Acetazolamide in the pharmaceutical tablet and serum samples. The dynamic range found for the determination of Acetazolamide concentration is 4.49 x 10(-9)-1.28 x 10(-7) mol L-1, and the limit of detection (LOD) and limit of quantification (LOQ) are (4.0 x 10(-9) and 1.21 x 10(-8)) mol L-1, respectively.

• 出版日期2018-4-15