Amniotic Fluid-Derived Mesenchymal Stem Cells Prevent Fibrosis and Preserve Renal Function in a Preclinical Porcine Model of Kidney Transplantation

作者：Baulier Edouard; Favreau Frederic; Le Corf Amelie; Jayle Christophe; Schneider Fabrice; Goujon Jean Michel; Feraud Olivier; Bennaceur Griscelli Annelise; Hauet Thierry^*; Turhan Ali G

来源：Stem Cells Translational Medicine, 2014, 3(7): 809-820.

DOI：10.5966/sctm.2013-0186

• Amniotic fluid-derived mesenchymal stem cells
• Kidney transplantation
• Pig
• Preclinical model
• Deceased after cardiac arrest donors
• Ischemia reperfusion injury

It is well known that ischemia/reperfusion injuries strongly affect the success of human organ transplantation. Development of interstitial fibrosis and tubular atrophy is the main deleterious phenomenon involved. Stem cells are a promising therapeutic tool already validated in various ischemic diseases. Amniotic fluid-derived mesenchymal stem cells (af-MSCs), a subpopulation of multipotent cells identified in amniotic fluid, are known to secrete growth factors and anti-inflammatory cytokines. In addition, these cells are easy to collect, present higher proliferation and self-renewal rates compared with other adult stem cells (ASCs), and are suitable for banking. Consequently, af-MSCs represent a promising source of stem cells for regenerative therapies in humans. To determine the efficiency and the safety of af-MSC infusion in a preclinical porcine model of renal autotransplantation, we injected autologous af-MSCs in the renal artery 6 days after transplantation. The af-MSC injection improved glomerular and tubular functions, leading to full renal function recovery and abrogated fibrosis development at 3 months. The strong proof of concept generated by this translational porcine model is a first step toward evaluation of af-MSC-based therapies in human kidney transplantation.

• 出版日期2014-7