Anti-inflammatory effects of artificial synthetic E-selectin on common carotid artery aneurysm in an elastase induced rabbit model

作者：Lu, Jun; Dai, Dawei; Zhang, Shiming^*; Wu, Jiang; Jiao, Lei; Huang, Yabo

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(12): 12595-12601.

• Aneurysm
• MCP-1
• model
• NF-kappa B
• artificial synthetic E-selectin

In the present study, we tested the effects of artificial synthetic E-selectin on expressions of NF-kappa B and MCP-1 in an elastase-induced rabbit model of common carotid artery aneurysm. 42 New Zealand rabbits (21 male and 21 female animals) were randomly divided into three (control, disease, and treatment) groups. The right common carotid artery was exposed and injected with either 0.2 ml of phosphate-buffered saline (control group) or elastase (disease and treatment groups). The rabbits in the treatment group received artificial synthetic E-selectin (1 mg/kg/day for up to 3 weeks) through a venous catheter. Morphologic changes of the right common carotid artery and aneurysm formation were assessed by CT angiography and hematoxylin & eosin staining. Specimens of aneurysm tissue were collected, and mRNA and protein expressions of NF-kappa B and MCP-1 were evaluated by, respectively, qPCR and immunohistochemistry. Compared with the disease group, animals treated with artificial synthetic E-selectin showed decreased dimensions of aneurysms, with the differences becoming significant starting from week 2 (P < 0.05). Furthermore, expressions of NF-kappa B and MCP-1 (both mRNA and protein) were decreased in treated animals. Artificial synthetic E-selectin inhibits expression of NF-kappa B and MCP-1 in an experimental model of common carotid artery aneurysm. Treatment with artificial synthetic E-selectin may attenuate aneurysm development.

• 出版日期2016
• 单位苏州大学