Approach to the Biosynthesis of Atisine-Type Diterpenoid Alkaloids

作者：Zhao Pei Ji; Gao Suo; Fan Li Ming; Nie Jing Lei; He Hong Ping; Zeng Ying; Shen Yue Mao; Hao Xiao Jiang^*

来源：Journal of Natural Products, 2009, 72(4): 645-649.

DOI：10.1021/np800657j

To determine the biosynthesis pathway of the atisine-type diterpenoid alkaloids spiramines A/B and C/D, feeding experiments in in vitro Cultured plantlets; and enzymatic transformations in cell-free extracts were performed in combination with LCMS and tandem MS analyses. L-[2-(13)C,(15)N]Serine was used in the feeding experiments and enzymatic transformations, and the diterpene spiraminol was identified as it biosynthetic precursor of spiramine alkaloids. The LCMS and tandem MS spectra of the extracts from these experiments indicated that L-[2-(13)C,(15)N]serine was incorporated into spiramines A/B and C/D. The labeled reaction products show that L-serine is the one possible nitrogen source involved in the biosynthesis of atisine-type DAs.

• 出版日期2009-4
• 单位中国科学院昆明植物研究所