A mechanism of transcriptional regulation of the CSF-1 gene by interferon-gamma

作者：Tsuchimoto D^*; Tojo A; Asano S

来源：Immunological Investigations, 2004, 33(4): 397-405.

DOI：10.1081/IMM-200038662

• interferon-gamma
• CSF-1
• cis-acting element
• GAS
• STAT1

Interferon (IFN)-gamma exerts multiple functions including antiviral, anti-proliferative and immunomodulatory activities, which are mediated through the JAK-STAT pathway. We observed that IFN-gamma significantly increases the production of colony stimulating factor-1 (CSF-1) by a human lung carcinoma cell line, A549. To gain insight into this mechanism, we determined the unknown nucleotide sequences of 5'-flanking region of human CSF-1 gene. About 60 bases upstream of the transcription start site of the CSF-1 gene contains a possible gamma interferon activated site (GAS), TTCCCATAA. The promoter assay and the electrophoretic mobility gel shift assay revealed that IFN-gamma stimulates transcription of the CSF-1 gene through this sequence, which binds STAT1.

• 出版日期2004
• 单位河北医科大学