Actin nucleation at the centrosome controls lymphocyte polarity

作者：Obino Dorian; Farina Francesca; Malbec Odile; Saez Pablo J; Maurin Mathieu; Gaillard Jeremie; Dingli Florent; Loew Damarys; Gautreau Alexis; Yuseff Maria Isabel; Blanchoin Laurent; Thery Manuel; Lennon Dumenil Ana Maria^*

来源：Nature Communications, 2016, 7(1): 10969.

DOI：10.1038/ncomms10969

Cell polarity is required for the functional specialization of many cell types including lymphocytes. A hallmark of cell polarity is the reorientation of the centrosome that allows repositioning of organelles and vesicles in an asymmetric fashion. The mechanisms underlying centrosome polarization are not fully understood. Here we found that in resting lymphocytes, centrosome-associated Arp2/3 locally nucleates F-actin, which is needed for centrosome tethering to the nucleus via the LINC complex. Upon lymphocyte activation, Arp2/3 is partially depleted from the centrosome as a result of its recruitment to the immune synapse. This leads to a reduction in F-actin nucleation at the centrosome and thereby allows its detachment from the nucleus and polarization to the synapse. Therefore, F-actin nucleation at the centrosome-regulated by the availability of the Arp2/3 complex-determines its capacity to polarize in response to external stimuli.

• 出版日期2016-3
• 单位中国地震局