A sericin-derived peptide protects Sf9 insect cells from death caused by acute serum deprivation

作者：Takahashi M^*; Tsujimoto K; Kato Y; Yamada H; Takagi H; Nakamori S

来源：Biotechnology Letters, 2005, 27(13): 893-897.

DOI：10.1007/s10529-005-7177-5

• bioactive peptide
• cell death
• sericin
• serum deprivation
• silk protein

Sericin is the silk protein enveloping fibroin fibers in cocoons. Sericin hydrolysate protects cultured Sf9 insect cells from death caused by serum deprivation; the activity depends on the repeats of 38 amino acids. A partial peptide from the 38 residues, SGGSSTYGYS, inhibited serum-deprivation death as well. Cell viabilities in the presence of 10% (v/v) foetal calf serum, no additives and 1 mM SGGSSTYGYS were 96, 12 and 31% on the third day after inoculation, respectively. Aromatic residues seemed to be important because SGGSSTWGWS had the same activity as SGGSSTYGYS but SGGSSTAGAS had no activity.

• 出版日期2005-7