Design, synthesis, structure information and biochemical activity of new floro substituted organotin(IV) carboxylates

作者：Shah Farooq Ali; Fatima Kaneez; Sabir Shaista; Ali Saqib; Qadri Ishtiaq^*; Din Noor Ud

来源：Journal of Photochemistry and Photobiology B: Biology , 2016, 154: 99-107.

DOI：10.1016/j.jphotobiol.2015.10.011

• Triorganotin(IV) compounds
• HCV
• IC50, luciferase assay
• DNA
• Binding energy constant

Four new triorganotin(IV) complexes with general formula R3SnL (R = C4H9, C6H5, and L = 3-[(fluorophenylamido)]propenoic acid, 3-[(fluorophenylamido)]propanoic acid) were synthesized and characterized by elemental analyses, FT-IR, NMR (H-1, C-13 and Sn-119), mass spectrometry and single crystal X-ray structural analysis. The disappearance of the OH peak of the carboxylic acid in the FT-IR and NMR spectra of the compounds conform the formation of the compound and suggests that the complexation occurs via oxygen atoms of the carboxylate moiety. FT-IR date shows the bidentate nature of the carboxylate moiety of the ligand as the Av value in all complexes is less than 250. Crystallographic data for compound 2 showed that tin has distorted tetrahedral geometry with 433.42 degrees angle around the central tin atom. The compounds (1-4) bind to DNA, resulting hypochromism shifts in UV-visible spectroscopy suggesting an intercalative mode of interactions. The compound-DNA interaction results (UV-visible and Viscometery) encourage using the compounds against HCV. The compounds (1-4) were screened for anti-HCV activity using Huh7.5 cell (human hepatoma cell) by the Gaussia Luciferase Assay and found to be biologically active. Based on Gaussia Luciferase Assay, compound 3 (Tributylstannic [3-(2-fluorophenylamido)propionatel) was taken for quantitative analysis by "QRT-PCR" using the serum of HCV patients and was found to have substantial anti-HCV activity. This work, demonstrated that compound 3 may be used as a potential anti-HCV agent in the future.

• 出版日期2016-1