Association between polymorphisms in microRNA target sites and survival in early-stage non-small cell lung cancer

作者：Yoo Seung Soo; Hong Mi Jeong; Lee Jang Hyuck; Choi Jin Eun; Lee Shin Yup; Lee Jaehee; Cha Seung Ick; Kim Chang Ho; Seok Yangki; Lee Eungbae; Cho Sukki; Jheon Sanghoon; Park Jae Yong^*

来源：Thoracic Cancer, 2017, 8(6): 682-686.

DOI：10.1111/1759-7714.12478

• miRNA target site
• non-small cell lung cancer
• polymorphism
• survival outcome

A high-throughput mapping method of RNA-RNA interactions by crosslinking, ligation, and sequencing of hybrids (CLASH) can not only provide information about canonical but also non-canonical interactions. We evaluated the associations between variants in microRNA target sites using CLASH data and survival outcomes of 782 early-stage non-small cell lung cancer (NSCLC) patients who underwent curative surgical resection. Among the 100 variants studied, two variants showed significant association with survival outcomes. The POLR2A rs2071504 C > T variant was associated with poor overall and disease-free survival under a dominant model (hazard ratio [HR]1.42, 95% confidence interval [CI]1.08-1.88; P=0.01 and HR1.34, 95% CI1.08-1.67; P=0.01, respectively). Patients carrying the NR2F6 rs2288539 TT genotype showed significantly better overall survival than those with the NR2F6 rs2288539 CC or CT genotypes (HR0.13, 95% CI0.02-0.90; P=0.04). These findings suggest that POLR2A rs2071504 C > T and NR2F6 rs2288539 C > T can influence prognosis in early-stage NSCLC patients.

• 出版日期2017-11