An optimized high-throughput fluorescence plate reader-based RSV neutralization assay

作者：Sun, Yong-Peng; Zhang, Wei; Zhao, Qin-Jian; Cao, Jian-Li; Zhang, Lu-Jing; Xiang, Jiang-Yan; Si, Jun-Yu; Lin, Xue; Chen, Li; Zheng, Zi-Zheng^*; Xia, Ning-Shao^*

来源：Journal of Virological Methods, 2018, 260: 34-40.

DOI：10.1016/j.jviromet.2018.07.004

• Fluorescence plate reader
• Flow cytometry
• RSV
• Neutralization assay

A licensed vaccine for respiratory syncytial virus (RSV) has yet to be developed, and a reliable and repeatable neutralizing assay is indispensable for vaccine development. Here, we demonstrated an optimized high throughput RSV neutralization assay that utilizes a fluorescence plate reader (reader) as a substitute for flow cytometry to detect fluorescent signals in RSV-A2 mKate-infected cells. Furthermore, this study tested the influence of virus input and infectivity on the neutralizing assay and highlighted critical factors (together with a suggested protocol) for obtaining stable data using this assay.

• 出版日期2018-10
• 单位厦门大学