Gold nanoparticles/horseradish peroxidase encapsulated polyelectrolyte nanocapsule for signal amplification in Listeria monocytogenes detection

作者：Oaew Sukunya^*; Charlermroj Ratthaphol; Pattarakankul Thiti****; Karoonuthaisiri Nitsara

来源：Biosensors and Bioelectronics, 2012, 34(1): 238-243.

DOI：10.1016/j.bios.2012.02.011

• Gold nanoparticles
• Horseradish peroxidase
• Polyelectrolyte
• Nanocapsule
• Encapsulation
• Listeria monocytogenes

Bioconjugate nanocapsules were fabricated by using polystyrene sulfonate (PSS) to encapsulate gold nanoparticles (AuNPs) bearing adsorbed horseradish peroxidase (HRP). The average size of nanocapsule was in a range 150-400 nm. The efficiency of the capsules to enhance signals in an immunoassay was demonstrated by using an enzyme linked immunosorbent assay (ELISA) to detect the food-borne pathogen - Listeria monocytogenes. The antibody adsorbed onto the PSS shell of the nanocapsules provided the recognition molecule. For a given quantity of antibody, the bioconjugate nanocapsules showed 30 times greater sensitivity and a shorter assay time (5 min) when compared to conventional ELM using an HRP labelled antibody. This proof-of-concept encapsulation of HRP through PSS nanocapsules may pave the way for alternative signal enhancement strategies where sensitivity is a priority.

• 出版日期2012-4-15