A novel bacterial transport mechanism of Acinetobacter baumannii via activated human neutrophils through interleukin-8

作者：Kamoshida Go; Tansho Nagakawa Shigeru; Kikuchi Ueda Takane; Nakano Ryuichi; Hikosaka Kenji; Nishida Satoshi; Ubagai Tsuneyuki; Higashi Shouichi; Ono Yasuo

来源：Journal of Leukocyte Biology, 2016, 100(6): 1405-1412.

DOI：10.1189/jlb.4AB0116-023RR

• adhesion
• in vitro transmigration assay
• invasion
• sepsis

Hospital-acquired infections as a result of Acinetobacter baumannii have become problematic because of high rates of drug resistance. Although neutrophils play a critical role in early protection against bacterial infection, their interactions with A. baumannii remain largely unknown. To elucidate the interactions between A. baumannii and human neutrophils, we cocultured these cells and analyzed them by microscopy and flow cytometry. We found that A. baumannii adhered to neutrophils. We next examined neutrophil and A. baumannii infiltration into Matrigel basement membranes by an in vitro transmigration assay. Neutrophilswere activated by A. baumannii, and invasion was enhanced. More interestingly, A. baumannii was transported together by infiltrating neutrophils. Furthermore, we observed by live cell imaging that A. baumannii and neutrophils moved together. In addition, A. baumannii-activated neutrophils showed increased IL-8 production. The transport of A. baumannii was suppressed by inhibiting neutrophil infiltration by blocking the effect of IL-8. A. baumannii appears to use neutrophils for transport by activating these cells via IL-8. In this study, we revealed a novel bacterial transport mechanism that A. baumannii exploits human neutrophils by adhering to and inducing IL-8 release for bacterial portage. This mechanism might be a new treatment target.

• 出版日期2016-12