A Y2H-seq approach defines the human protein methyltransferase interactome

作者：Weimann Mareike; Grossmann Arndt; Woodsmith Jonathan; Oezkan Ziya; Birth Petra; Meierhofer David; Benlasfer Nouhad; Valovka Taras; Timmermann Bernd; Wanker Erich E; Sauer Sascha; Stelzl Ulrich^*

来源：Nature Methods, 2013, 10(4): 339-+.

DOI：10.1038/NMETH.2397

To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and validated through coimmunoprecipitation experiments and defines previously undiscovered cellular roles of nonhistone protein methylation.

• 出版日期2013-4