Appropriate solvents for extracting total phenolics, flavonoids and ascorbic acid from different kinds of millets

作者：Upadhyay Rohit; Jha Alok^*; Singh Satyendra P; Kumar Arvind; Singh Mithilesh

来源：Journal of Food Science and Technology-Mysore, 2015, 52(1): 472-478.

DOI：10.1007/s13197-013-0976-0

• Millets
• Bajra
• Jowar
• Kodo
• Sawan
• Phenolic compounds
• Antioxidant activity

Different polar solvents (methanol, acetone and water) were tested for their extractability of phenolic compounds from millets grown in India namely, sawan (Echinochloa frumentacea), kodo (Paspalum scrobiculatum), jowar (Sorghum bicolor) and bajra (Pannisetum typhoides). The extracts were evaluated for their total phenolics, flavonoids, ascorbic acid content and antioxidant activity. The acetone extracts reported highest total phenolics content (TPC) between 0.8 and 2.64 mg gallic acid equivalents/g flour dry matter; the lowest for sawan and the highest for bajra. Ascorbic acid content did not differ significantly between different millets (p < 0.05) except for sawan (lowest). The antioxidant assays included 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (OH*) scavenging and reducing power. The activity in millets followed as bajra > jowar > kodo > sawan. The polyphenols in extracts were also analyzed by high-performance liquid chromatography (HPLC) at 290 nm. A clear correlation obtained between the TPC, flavonoids content and antioxidant activity. Acetone showed better extractability and thus can be appropriate solvent to extract phenolic compounds in millets used in this study.

• 出版日期2015-1