Apoptosis-related mRNA expression profiles of ovarian cancer cell lines following cisplatin treatment

作者：Yoon JooHee; Kim Eung Sam; Lee Sung Jong; Park Chang Wook; Cha Hyung Jin; Hong Bee Hak; Choi Kwan Yong^*

来源：Journal of Gynecologic Oncology, 2010, 21(4): 255-261.

DOI：10.3802/jgo.2010.21.4.255

• Ovarian cancer cell lines
• Cisplatin
• Apoptosis
• Dendron-modified DNA microarray

Objective: The aim of this study was to identify apoptosis-related genes of ovarian cancer cell lines following cisplatin treatment.
Methods: We used IC(50) values and fluorescence-activated cell sorting analysis to compare cell death in 2 ovarian cancer cell lines, namely, SKOV-3 and OVCAR-3, upon treatment with cisplatin. Moreover, the change in transcriptional levels of apoptosis-associated genes was measured with a dendron-modified DNA microarray.
Results: The protein levels for the up-regulated genes in each cell line were validated to identify the molecules that may determine the cellular behavior of cisplatin resistance. Eight genes were over-expressed in the 2 cell lines. The cisplatin-induced up-regulation of DAD1 in transcriptional and protein levels contributed to the cisplatin resistance of OVCAR-3, and the up-regulation of FASTK and TNFRSF11A in SKOV-3 resulted in its higher sensitivity to cisplatin than that of OVCAR-3.
Conclusion: In the present study, we have identified a set of genes responsible for apoptosis following cisplatin treatment in ovarian cancer cell lines. These genes may give information about the understanding of cisplatin-induced apoptosis in ovarian cancer.

• 出版日期2010-12