GROWTH-FACTOR AND PROCOLLAGEN TYPE-I GENE-EXPRESSION IN HUMAN LIVER-DISEASE

作者：MALIZIA G^*; BRUNT EM; PETERS MG; RIZZO A; BROEKELMANN TJ; MCDONALD JA

来源：Gastroenterology, 1995, 108(1): 145-156.

DOI：10.1016/0016-5085(95)90019-5

Background/Aims: Growth factors have been implicated in the pathogenesis of liver fibrosis, a major determinant of the clinical course of chronic liver disease, The aim of this study was to study the relationship of growth factor expression to inflammation and fibrosis in a variety of human liver diseases, Methods: We studied by in situ hybridization the expression of transforming growth factor (TGF) beta 1, platelet-derived growth factor (PDGF) A and PDGF-B, and procollagen type I (pro-I) messenger RNAs (mRNAs) in liver diseases of various etiologies. Results: Pro-I mRNA was expressed by mesenchymal cells at sites of inflammation and scarring, where TGF-beta 1 immunoreactivity was often found, and by perisinusoidal cells, TGF-beta 1 and PDGF-A mRNAs were expressed mainly by mononuclear cells and proliferating ductular cells. TGF-beta 1 mRNA was also expressed by perisinusoidal cells, PDGF-A gene expression was more common than that of PDGF-B, Pro-I and TGF-beta 1 expression correlated with both ductular proliferation and tissue inflammation, whereas PDGF-A and PDGF-B only correlated with ductular proliferation. Conclusions: Our data suggest that TGF-beta 1 and PDGF are involved in human liver inflammation and fibrosis. The expression of growth factor mRNAs in proliferating ductular cells may indicate a role for these cells in liver fibrogenesis and may help explain the pathophysiology of conditions such as biliary atresia progressing to fibrosis despite the absence of marked inflammation.

• 出版日期1995-1