Applications of Fluorescence Correlation Spectroscopy to the Study of Nucleic Acid Conformational Dynamics

作者:Gurunathan Kaushik*; Levitus Marcia
来源:PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 82, ELSEVIER ACADEMIC PRESS INC, 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA, 33-69, 2008.
DOI:10.1016/S0079-6603(08)00002-0

摘要

Fluorescence correlation spectroscopy (FCS) is a quantitative technique where temporal fluctuations in fluorescence intensity are analyzed to yield information about physical processes that contribute to the fluctuations, These include diffusion through the observation volume, conformational dynamics, and chemical or photophysical reactions. To date, FCS applications in biochemistry and biophysics have been mostly focused on the investigation of molecular diffusion. In this chapter, we present a look into the recent applications of FCS to the study of conformational dynamics of nucleic acids. We first survey the basic theoretical and experimental aspects of the technique and then focus oil the results obtained in studies with DNA hairpins, nucleosomes, and single-stranded and duplex DNA.

  • 出版日期2008