Miniaturized capillary electrophoresis system with slotted micro-vial array based sample introduction system and ultraviolet detection

作者：Liu J; Fang Q^*; Du WB

来源：Chinese Journal of Analytical Chemistry, 2005, 33(12): 1799-1802.

• capillary electrophoresis
• sample introduction
• ultraviolet detection

A high throughput sample introduction system that based on slotted micro-vial array coupled with a miniaturized capillary electrophoresis (CE) system with short capillary and ultraviolet detection to achieve automated and continuous sample introduction and separation was developed. The sample introduction system is composed of an array of horizonally positioned micro-sample vials with slits fabricated on the bottom of each vial. Sample introduction was performed by linearly moving the array of vials, allowing the capillary and electrode to sequentially enter the solutions in the vials through the slits achieving sample introduction and separation operation. The present system was applied in fast separation of sulphamethoxazole (SMZ) and trimethoprim (TMP) in sulphatrim tablets, achieving a high throughput of 72/h and a low carryover of 1.4%. Separation efficiency of 11 mu m plate height for SMZ was obtained. The detection limits(3 sigma) for SMZ and TMP were 9.8 mg/L and 12.2 mg/L, respectively.

• 出版日期2005-12
• 单位浙江大学