摘要
We investigated whether Nd2O3 treatment results in cytotoxicity and other underlying effects in rat NR8383 alveolar macrophages. Cell viability assessed by the MTT assay revealed that Nd2O3 was toxic in a dose-dependent manner, but not in a time-dependent manner. An ELISA analysis indicated that exposure to Nd2O3 caused cell damage and enhanced synthesis and release of inflammatory chemokines. A Western blot analysis showed that protein expression levels of caspase-3, nuclear factor-kappa B (NF-kappa B) and its inhibitor I kappa B increased significantly in response to Nd2O3 treatment. Both NF-kappa B and caspase-3 signaling were activated, suggesting that both pathways are involved in Nd2O3 cytotoxicity.
- 出版日期2017-1
- 单位内蒙古科技大学包头医学院