An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

作者：Corces, M. Ryan; Trevino, Alexandro E.; Hamilton, Emily G.; Greenside, Peyton G.; Sinnott-Armstrong, Nicholas A.; Vesuna, Sam; Satpathy, Ansuman T.; Rubin, Adam J.; Montine, Kathleen S.; Wu, Beijing; Kathiria, Arwa; Cho, Seung Woo; Mumbach, Maxwell R.; Carter, Ava C.; Kasowski, Maya; Orloff, Lisa A.; Risca, Viviana I.; Kundaje, Anshul; Khavari, Paul A.; Montine, Thomas J.; Greenleaf, William J.^*; Chang, Howard Y.^*

来源：Nature Methods, 2017, 14(10): 959-+.

DOI：10.1038/NMETH.4396

• OPEN CHROMATIN
• LANDSCAPE
• ACCESSIBILITY
• DISEASE
• INNATE
• CELLS

We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-mu m sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.

• 出版日期2017-10