Macrophage-Dependent Cytoplasmic Transfer during Melanoma Invasion In Vivo

作者：Roh Johnson Minna^*; Shah Arish N; Stonick Jason A; Poudel Kumud R; Kargl Julia; Yang Grace H; di Martino Julie; Hernandez Rafael E; Gast Charles E; Zarour Luai R; Antoku Susumu; Houghton A McGarry; Bravo Cordero Jose Javier; Wong Melissa H; Condeelis John; Moens Cecilia B

来源：Developmental Cell, 2017, 43(5): 549-+.

DOI：10.1016/j.devcel.2017.11.003

Interactions between tumor cells and tumor-associated macrophages play critical roles in the initiation of tumor cell motility. To capture the cellular interactions of the tumor microenvironmentwith high-resolution imaging, we directly visualized tumor cells and their interactions with macrophages in zebrafish. Live imaging in zebrafish revealed that macrophages are dynamic, yet maintain sustained contact with tumor cells. In addition, the recruitment of macrophages to tumor cells promotes tumor cell dissemination. Using a Cre/LoxP strategy, we found that macrophages transfer cytoplasm to tumor cells in zebrafish and mouse models. Remarkably, macrophage cytoplasmic transfer correlated with melanoma cell dissemination. We further found that macrophages transfer cytoplasm to tumor cells upon cell contact in vitro. Thus, we present a model in which macrophage/tumor cell contact allows for the transfer of cytoplasmic molecules from macrophages to tumor cells corresponding to increased tumor cell motility and dissemination.

• 出版日期2017-12-4