Neuroprotective effect of morroniside on focal cerebral ischemia in rats

作者：Wang Wen^*; Xu Jingdong; Li Lei; Wang Peichang; Ji Xunming; Ai Houxi; Zhang Li; Li Lin

来源：Brain Research Bulletin, 2010, 83(5): 196-201.

DOI：10.1016/j.rainresbull.2010.07.003

• Anti apoptotic
• Antioxidant
• Cerebral ischemia
• Middle cerebral artery occlusion
• Morroniside
• Neuroprotection

Cornus officinalis Sieb et Zucc known as Shan-zhu-yu in Chinese has been used to treat cerebrovascular disease and diabetes in Traditional Chinese Medicine for a long time and morroniside is the main component of Shan-zhu-yu In this study we examined whether morroniside could protect ischemia/reperfusion-induced brain injury by minimizing oxidative stress and anti-apoptosis Morroniside was intragastrically administered to rats in doses of 30 90 and 270 mg/kg/day starting 3 h after the onset of middle cerebral artery occlusion The behavioral test was performed by using the Zea-Longa scores Prehensile Traction score and Ludmila Belayer score Rats were sacrificed 3 days after ischemia occurred The infarction volume of brain was assessed in the brain slices stained with 2 3 5-triphenyl tetrazolium chloride Cortex tissues were also used for determination of malondialdehyde levels glutathione levels and superoxide dismutase The treatment with morroniside significantly improved Zea-Longa scores and Prehensile Traction score at the doses of 30 90 and 270 mg/kg increased Ludmila Belayer score and reduced the infarction volume at the doses of 90 and 270 mg/kg Morroniside (30 90 and 270 mg/kg) treatment significantly decreased the level of malondialdehyde and caspase-3 activity by colorimetric analysis in ischemic cortex tissues Morroniside (270 mg/kg) treatment significantly Increased the content of glutathione enhanced the activity of superoxide dismutase but decreased the caspase-3 expression by Western-blot analysis in ischemic cortex tissues These findings demonstrated that morroniside could notably

• 出版日期2010-10-30
• 单位首都医科大学