Antioxidant and nitric oxide release inhibition activities of methanolic extract from Clerodendrum cyrtophyllum Turcz

作者：Liu Huan; Wang Lan; Wang Myeong Hyeon^*

来源：Horticulture Environment and Biotechnology, 2011, 52(3): 309-314.

DOI：10.1007/s13580-011-0204-7

• 1,1-diphenyl-2-picrylhydrazyl (DPPH)
• anti-inflammatory
• DNA damage
• protein damage
• semi-quantitative RT-PCR

Antioxidant and anti-inflammatory activities of methanolic extract of Clerodendrum cyrtophyllum Turcz (ECT) were investigated in the present study. ECT showed high phenolic content (223 mu g center dot GAE(-1) equivalent) and strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (IC(50) = 52.74 mu g center dot mL(-1)) and reduced power ability. Furthermore, ECT exhibited significant protective ability in H(2)O(2)/Fe(3+) /ascorbic acid-induced DNA and protein damage. Moreover, ECT inhibited NO production by 47.15% at 0.8 mg center dot mL(-1) in lipopolysaccharides-stimulated RAW264.7 macrophages. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis showed that the expression of iNOS and COX-2 in RAW264.7 cells was down-regulated after treatment with increasing concentration of ECT. In conclusion, ECT has shown potent antioxidant and anti-inflammatory activities could be considered as a source of health promoting antioxidant and complementary medicinal supplement.

• 出版日期2011-6