A Fluorescent Polymer Probe with High Selectivity toward Vascular Endothelial Cells for and beyond Noninvasive Two-Photon Intravital Imaging of Brain Vasculature

作者：Mettra B; Appaix F; Olesiak Banska J; Le Bahers T; Leung A; Matczyszyn K; Samoc M; van der Sanden B; Monnereau C^*; Andraud C^*

来源：ACS Applied Materials & Interfaces, 2016, 8(27): 17047-17059.

DOI：10.1021/acsami.6b02936

• star-shaped polymer
• fluorescence
• near infrared
• two photon absorption
• intravital microscopy
• TD-DFT

A chromophore-engineering strategy that relies on the introduction of a ground-state distortion in a quadrupolar chromophore was used to obtain a quasi-quadrupolar chromophore with red emission and large two-photon absorption (2PA) cross-section in polar solvents. This molecule was functionalized with water-solubilizing polymer chains. It constitutes not only a remarkable contrast agent for intravital two-photon microscopy of the functional cerebral vasculature in a minimally invasive configuration but presents intriguing endothelial staining ability that makes it a valuable probe for premortem histological staining.

• 出版日期2016-7-13