APE1/Ref-1 as a Seroloqical Biomarker for the Detection of Bladder Cancer

作者：Shin Ju Hyun; Choi Sunga; Lee Yu Ran; Park Myoung Soo; Na Yong Gil; Irani Kaikobad; Lee Sang Do; Park Jin Bong; Kim Jin Man; Lim Jae Sung; Jeon Byeong Hwa^*

来源：Cancer Research and Treatment, 2015, 47(4): 823-833.

DOI：10.4143/crt.2014.074

• Apurinic/apyrimidinic endonuclease 1/redox factor-1
• Urinary bladder neoplasms
• Biological markers
• Enzyme linked immunosorbent assay

Purpose
Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a multifunctional protein that shows elevated expression in a number of cancers. We attempted to determine whether serum APE1/Ref-1 is elevated in patients with bladder cancer.
Materials and Methods
Serum APE1/Ref-1 levels were determined using enzyme linked immunosorbent assay in serum from patients with bladder cancer who had not received chemotherapy or radiotherapy (n=51) and non-tumor controls (n=55). The area under the receiver operating characteristic area under the curve was applied to determine the correlation between clinical factors and the serum levels of APE1/Ref-1.
Results
Serum levels of APE1/Ref-1 in bladder cancer patients were significantly elevated compared to those of the control group (3.548 0.333 ng/100 pL [n=511 for bladder cancer vs. 1.547 0.319 ng/100 pL [n=55] for the control group), with a sensitivity and specificity of 93% and 59%, respectively. Serum APE1/Ref-1 levels are associated with tumor stage, grade, muscle invasion, and recurrence.
Conclusion
Serum APE1/Ref-1 might be useful as a potential serologic biomarker for bladder cancer.

• 出版日期2015-10
• 单位河北医科大学