Highly sensitive and selective aptasensor for detection of adenosine based on fluorescence resonance energy transfer from carbon dots to nano-graphite

作者：Wang, Xu; Xu, Guanhong; Wei, Fangdi; Ma, Yunsu; Ma, Yujie; Song, Yueyue; Cen, Yao; Hu, Qin^*

来源：Journal of Colloid and Interface Science, 2017, 508: 455-461.

DOI：10.1016/j.jcis.2017.07.028

• Carbon dots
• Aptamer
• Adenosine
• Nano-graphite
• Fluorescence resonance energy transfer

In this article, a novel aptasensor was fabricated by modifying carbon dots (CDs) with adenosine aptamer (CDs-aptamer) for sensitive, selective and quantitative detection of adenosine (AD). When nano-graphite (NG) as an energy acceptor was added into the CDs-aptamer (energy donor) solution, the fluorescence of CDs-aptamer was quenched due to fluorescence resonance energy transfer (FRET). When AD was present in the solution of CDs-aptamer/NG, the process of FRET was inhibited because of the specific combination between AD and AD aptamer. As a result, the fluorescence of CDs-aptamer was restored due to the dissociation of CDs-aptamer from NG and its change was proportional to the AD concentration. Under the optimized conditions, a linear range was found to be 2-50 nM for the detection of AD with a detection limit of 0.63 nM. Furthermore, the application of the proposed approach was demonstrated in real sample with satisfying results and it showed promise in diagnostic purpose.

• 出版日期2017-12-15
• 单位南京医科大学