New Transcriptional Reporters to Quantify and Monitor PPARγ Activity

作者：Séverine A., Degrelle; Hussein, Shoaito; Thierry, Fournier

来源：PPAR Research, 2017, 2017: 1-7.

DOI：10.1155/2017/6139107

<jats:p>The peroxisome-proliferator-activated-receptor-<jats:italic>γ</jats:italic> (PPAR<jats:italic>γ</jats:italic>) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPAR<jats:italic>γ</jats:italic>, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPAR<jats:italic>γ</jats:italic> activity in different cell types and screen for PPAR<jats:italic>γ</jats:italic>’s potential ligands.</jats:p>

• 出版日期2017
• 单位常州工学院