茶多酚抑制环孢菌素A诱导的慢性肾毒性大鼠转化生长因子-β1的表达Inhibitory effect of tea polyphenals on transforming growth factor-β1 expression in rat with cyclosporine A-induced chronic nephrotoxicity

作者：施邵华; 郑树森; 贾长库; 朱有法; 谢海洋

来源：Acta Pharmacologica Sinica, 2004, 25(1): 98-103.

• tea
• cyclosporine A
• transforming growth factor-β
• kidney
• polymerase chain reaction
• immunohistochemistry
• Western blotting

AIM: To investigate the inhibitory effect of tea polyphenols (TP) on the transforming growth factor-β1 (TGF-β1)expression in rat model of cyclosporine A (CsA)-induced chronic nephrotoxicity. METHODS: The rat model of CsA-induced chronic nephrotoxicity was used, 4 groups of rats were respectively treated with vehicle (0.1 mL .kg-1.d-1 At the end of day 28 of treatment, serum and urine are analyzed for creatinine clearance, kidney tissue for pathologic analysis. The TGF-β1 mRNA and its protein expression were detected by RT-PCR, immunohistochemistry,and Western blot. RESULTS: CsA-treated rats had increased renal expression of TGF-βl mRNA and its protein,compared with the vehicle- or TP- treated controls. The renal function and interstitial fibrosis were ameliorated and renal expression of TGF-β1 mRNA and its protein was decreased in animals treated with CsA plus TP, compared with aminals treated with CsA alone (P＜0.05). CONCLUSION: TP significantly inhibits renal expression of TGFβ1 in rat model of cyclosporine-induced chronic nephrotoxicity, suggesting that the decreased renal expression of TGF-β1 exerted by TP is one of mechanisms to protect renal function and tissue structure.

• 出版日期2004
• 单位浙江大学