A Novel Mitosomal beta-Barrel Outer Membrane Protein in Entamoeba

作者：Santos Herbert J; Imai Kenichiro; Makiuchi Takashi; Tomii Kentaro; Horton Paul; Nozawa Akira; Ibrahim Mohamed; Tozawa Yuzuru; Nozaki Tomoyoshi^*

来源：Scientific Reports, 2015, 5(1): 8545.

DOI：10.1038/srep08545

Entamoeba possesses a highly divergent mitochondrion-related organelle known as the mitosome. Here, we report the discovery of a novel protein in Entamoeba, which we name Mitosomal beta-barrel Outer Membrane Protein of 30 kDa (MBOMP30). Initially identified through in silico analysis, we experimentally confirmed that MBOMP30 is indeed a beta-barrel protein. Circular dichroism analysis showed MBOMP30 has a predominant beta-sheet structure. Localization to Entamoeba histolytica mitosomes was observed through Percoll-gradient fractionation and immunofluorescence assay. Mitosomal membrane integration was demonstrated by carbonate fractionation, proteinase K digestion, and immunoelectron microscopy. Interestingly, the deletion of the putative beta-signal, a sequence believed to guide beta-barrel outer membrane protein (BOMP) assembly, did not affect membrane integration, but abolished the formation of a,240 kDa complex. MBOMP30 represents only the seventh subclass of eukaryotic BOMPs discovered to date and lacks detectable homologs outside Entamoeba, suggesting that it may be unique to Entamoeba mitosomes.

• 出版日期2015-2-25