Multifunctional mesoporous silica nanoparticles as sensitive labels for immunoassay of human chorionic gonadotropin

作者：Wei Qin; Li Ru; Du Bin; Wu Dan; Han Yanyan; Cai Yanyan; Zhao Yanfang; Xin Xiaodong; Li He^*; Yang Minghui

来源：Sensors and Actuators B: Chemical , 2011, 153(1): 256-260.

DOI：10.1016/j.snb.2010.09.073

• Multifunctional mesoporous silica naonnoparticles
• Platinum nanoparticles
• Human chorionic gonadotropin
• Immunoassay

A novel method to construct amperometric immunosensor for human serum chorionic gonadotrophin (hCG) has been described. In this study, horseradish peroxidase (HRP), Pt nanoparticles and secondary antibody (Ab(2)) modified MSN (Pt@MSN/HRP/Ab(2)) was synthesized and the multifunctional MSN was used as label for the preparation of immunosensor. With the hCG primary antibody immobilized onto thionine/graphene modified glassy carbon electrode (GCE) via crosslinking with glutaraldehyde, the electrochemical immunosensor was able to realize a reliable determination of hCG in the range of 0.01-12 ng mL(-1) with a detection limit of 7.50 pg mL(-1). This immunoassay system has many desirable merits including high sensitivity, accuracy, and little instrumentation requirement. Significantly, the new method may be quite promising, with potentially broad applications for clinical immunoassays.

• 出版日期2011-3-31
• 单位济南大学