科研之友
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摘要
Pulmonary fibrosis (PF) is a progressive, chronic, irreversible and life-threatening disease. In our research, we aimed to investigate the effects of Saikosaponin D (SSD) on pulmonary fibrosis by using bleomycin induced PF mice and human embryonic lung fibroblast (HELF). After successful preparation of BLM (5 mg/kg, intratracheal instillation) induced PF mice, SSD was administered to the BLM induced mice by intraperitoneal injection (2 mg/kg/d, ip) for 28 days. Then, lung tissues were collected for histological examination with H&E, Masson's trichrome and TUNEL staining. In addition, reverse transcription PCR assay was performed to determine the mRNA expression of Caspase-3, and western blotting was carried out to determine the protein expressions of E-cadherin (E-cad), fibronectin (FN), Wnt and beta-catenin. Furthermore, we also determined the anti-proliferative effects of SSD on HELF cells and transforming growth factor (TGF)-beta 1 expressions in HELF cells. Our results showed that SSD alleviated pulmonary alveolitis (P < 0.05), pulmonary fibrosis (P < 0.05) and cell apoptosis (P < 0.01) in BLM induced mice. Furthermore, SSD down-regulated Caspase-3 (P < 0.05), FN (P < 0.05), Wnt (P < 0.05) and beta-catenin (P < 0.05) in both 14 and 28 days, whereas the E-cad obviously up-regulated (P < 0.05). Besides, SSD also inhibited cell proliferation of HELF and the TGF-beta 1 (P < 0.05) expression. In conclusion, our research suggested that SSD possess notable anti-fibrosis effect of on PF via suppressing alveolar epithelial cell apoptosis and epithelium-mesenchymal transformation.
- 出版日期2017
- 单位苏州大学
